About Proline
L-Proline is a non-essential amino acid and a major component of collagen (~12% by composition). Hydroxylated proline (hydroxyproline) is critical for collagen triple helix stability. Supplementation rationale is based on providing substrate for collagen synthesis, but direct RCT evidence for oral proline supplementation on skin, joint, or wound healing outcomes is very limited. Most benefits attributed to proline are inferred from collagen peptide studies rather than free-form proline. The body can synthesize proline from glutamate.
What Proline supports
- Major building block of collagen structure
- Supports connective tissue and joint health
How much Proline to take
The RDA prevents deficiency. The effective range is what clinical trials used to actually move the outcome.
Effective
500–2000
mg
No well-defined clinical dose from RCTs. Supplemental doses typically 500–2000 mg/day based on collagen synthesis rationale.
Clinical evidence
Limited clinical evidence. No direct clinical trials for oral proline supplements; benefits inferred from collagen peptide studies
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