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Supplement

Bacillus subtilis

Evidence

Moderate
Evidence: 3 of 5 (Moderate)

What the evidence says

A spore-forming (soil-based) probiotic. The DE111 strain has RCTs showing survival/germination in the small intestine and immunomodulatory and GI effects in healthy adults. Note: this is distinct from Bacillus subtilis natto used to produce nattokinase (separate entry).

Moderate: DE111 has human RCTs for GI/immune outcomes.

Top Bacillus subtilis supplements

About Bacillus subtilis

A spore-forming (soil-based) probiotic. The DE111 strain has RCTs showing survival/germination in the small intestine and immunomodulatory and GI effects in healthy adults. Note: this is distinct from Bacillus subtilis natto used to produce nattokinase (separate entry). Strain-specific evidence.

What Bacillus subtilis supports

  • Spore form survives stomach acid and germinates in the gut
  • DE111 has moderate RCT evidence for GI and immune support

How much Bacillus subtilis to take

The RDA prevents deficiency. The effective range is what clinical trials used to actually move the outcome.

Effective

15

billion_cfu

DE111 RCTs use ~1-5 billion CFU/day; spore-forming so dose is lower than typical lactic-acid probiotics. Often presence-only in blends; nominal range.

Clinical evidence

Moderate clinical evidence. Moderate: DE111 has human RCTs for GI/immune outcomes.

NIH Fact Sheet